Review



alix  (Santa Cruz Biotechnology)


Bioz Verified Symbol Santa Cruz Biotechnology is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    Santa Cruz Biotechnology alix
    Alix, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Alix/pmc12874330-260-38-42
    Average 96 stars, based on 445 article reviews
    alix - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Milk:

    Article Title: Anti-oxidant and anti-inflammatory Effects of Aerosolised microalgal-derived extracellular vesicles in Bronchial Epithelial–Macrophage Co-cultures at the Air-Liquid Interface
    Article Snippet: .. Primary antibodies against H+-ATPase (dil. 1:1000 in in 5% Milk/TBS-T1X, Agrisera), Alix (clone 3A9, dil. 1:150 in in 3% BSA/TBS-T1X, Santa Cruz), were incubated overnight at 4°C and 2 h at room temperature. .. After washing steps, membranes were incubated for 1 h with species-specific HRP-conjugated secondary antibodies (Cell Signaling).

    Incubation:

    Article Title: Anti-oxidant and anti-inflammatory Effects of Aerosolised microalgal-derived extracellular vesicles in Bronchial Epithelial–Macrophage Co-cultures at the Air-Liquid Interface
    Article Snippet: .. Primary antibodies against H+-ATPase (dil. 1:1000 in in 5% Milk/TBS-T1X, Agrisera), Alix (clone 3A9, dil. 1:150 in in 3% BSA/TBS-T1X, Santa Cruz), were incubated overnight at 4°C and 2 h at room temperature. .. After washing steps, membranes were incubated for 1 h with species-specific HRP-conjugated secondary antibodies (Cell Signaling).

    Article Title: Engineered microalgal extracellular vesicles for efficient doxorubicin delivery and improved therapeutic efficacy in breast cancer
    Article Snippet: .. Proteins were separated, transferred to PVDF membranes, and blocked with 3 % BSA in TBS-T. Membranes were incubated overnight at 4 °C with primary antibodies against against H + -ATPase (1:1000, Agrisera), Enolase (clone A5, 1:150, Santa Cruz), Alix (clone 3A9, 1:150, Santa Cruz), and plant-actin (clone 10-B3, 1:500, Sigma-Aldrich), all diluted in blocking solution, were incubated overnight at 4 °C and 2h at room temperature, followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Cell Signaling). .. Detection was carried out using SuperSignal Pierce ECL (Thermo Fisher Scientific).

    Blocking Assay:

    Article Title: Engineered microalgal extracellular vesicles for efficient doxorubicin delivery and improved therapeutic efficacy in breast cancer
    Article Snippet: .. Proteins were separated, transferred to PVDF membranes, and blocked with 3 % BSA in TBS-T. Membranes were incubated overnight at 4 °C with primary antibodies against against H + -ATPase (1:1000, Agrisera), Enolase (clone A5, 1:150, Santa Cruz), Alix (clone 3A9, 1:150, Santa Cruz), and plant-actin (clone 10-B3, 1:500, Sigma-Aldrich), all diluted in blocking solution, were incubated overnight at 4 °C and 2h at room temperature, followed by incubation with horseradish peroxidase-conjugated secondary antibodies (Cell Signaling). .. Detection was carried out using SuperSignal Pierce ECL (Thermo Fisher Scientific).

    Western Blot:

    Article Title: Tetrastigma hemsleyanum alleviates febrile seizures by inhibiting HSPB1/Akt/NF-κB signaling via small extracellular vesicle-mediated astrocyte-microglia crosstalk
    Article Snippet: Background: Febrile seizures (FS) induced by hyperthermia are typically accompanied by inflammation, but how heat induces an inflammatory response is still unclear.. Methods and Results: We found pretreatment with total flavonoids from T. hemsleyanum (FTH) containing 36 components could inhibit FS in rats.. Microglia and astrocytes emerged as the two most prominent cell populations exhibiting significantly elevated heat shock protein B1 (Hspb1) gene expression after FS in the scRNA-seq data.

    Negative Control:

    Article Title: Chronic in utero oxycodone exposure alters placental small EV proteome and fetal cardiomyopathy-linked pathways
    Article Snippet: Following electrophoresis, proteins were transferred onto nitrocellulose membranes using iBlot2 (Invitrogen) and blocked in 5% non-fat dry milk in TBST. .. The following primary antibodies were used: Alix (C-11, sc-271975, Santa Cruz Biotechnology, USA), Annexin II (ab214486, Abcam, UK), CD81 (MCA1846, Bio-Rad Laboratories, Italy), and GM130 (610822, BD Biosciences, USA) as a negative control for cellular contamination. ..



    Similar Products

    ev markers alix  (Cell Signaling Technology Inc)
    96
    Cell Signaling Technology Inc ev markers alix
    Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins <t>(ALIX,</t> TSG101, <t>and</t> <t>CD81)</t> and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.
    Ev Markers Alix, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Alix+Rabbit+mAb/pmc12933830-100-6-10
    Average 96 stars, based on 1 article reviews
    ev markers alix - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    alix  (Proteintech)
    96
    Proteintech alix
    Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins <t>(ALIX,</t> TSG101, <t>and</t> <t>CD81)</t> and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.
    Alix, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Alix+Polyclonal+antibody/pmc13038339-74-61-66
    Average 96 stars, based on 1 article reviews
    alix - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    rabbit polyclonal alix  (Wanleibio)
    86
    Wanleibio rabbit polyclonal alix
    Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins <t>(ALIX,</t> TSG101, <t>and</t> <t>CD81)</t> and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.
    Rabbit Polyclonal Alix, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/anti+anti+mpo+myeloperoxidase+rabbit/pm42032459-97-32-36
    Average 86 stars, based on 1 article reviews
    rabbit polyclonal alix - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    anti albumin  (Bio-Rad)
    93
    Bio-Rad anti albumin
    Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins <t>(ALIX,</t> TSG101, <t>and</t> <t>CD81)</t> and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.
    Anti Albumin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Mouse+anti+ALIX/pm41999751-273-20-22
    Average 93 stars, based on 1 article reviews
    anti albumin - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    alix bio rad mca2493  (Bio-Rad)
    93
    Bio-Rad alix bio rad mca2493
    Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins <t>(ALIX,</t> TSG101, <t>and</t> <t>CD81)</t> and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.
    Alix Bio Rad Mca2493, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Mouse+anti+ALIX/pm41999751-207-52-53
    Average 93 stars, based on 1 article reviews
    alix bio rad mca2493 - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    anti alix  (Bio-Rad)
    93
    Bio-Rad anti alix
    Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins <t>(ALIX,</t> TSG101, <t>and</t> <t>CD81)</t> and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.
    Anti Alix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Mouse+anti+ALIX/pm41999751-273-11-13
    Average 93 stars, based on 1 article reviews
    anti alix - by Bioz Stars, 2026-09
    93/100 stars
    		  Buy from Supplier
    alix  (R&D Systems)
    94
    R&D Systems alix
    Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. <t>ALIX,</t> ALG‐2‐interacting protein X; ApoA1, apolipoprotein <t>A1;</t> <t>CD63,</t> cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.
    Alix, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Human+CD63+Antibody/pmc13069491-65-25-26
    Average 94 stars, based on 1 article reviews
    alix - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    transport escrt adaptor alix  (Galectin Therapeutics)
    86
    Galectin Therapeutics transport escrt adaptor alix
    ( A ) The <t>ESCRT</t> machinery, recruited by galectin-3 (Gal3) and <t>ALIX,</t> polymerizes at rupture sites to reseal small pores. ( B ) The PI4K2A/ORP/ATG9A lipid-transfer axis forms ER-lysosome bridges that restore membrane lipid composition and curvature. ATG2 cooperates with ATG9A vesicles to deliver lipids for bilayer expansion. ( C ) Neutral sphingomyelinase–dependent conversion of sphingomyelin to ceramide promotes local fusion and sealing. ( D ) Under severe stress, protein-RNA condensates assemble into transient stress granule patches that shield lesions until structural repair completes. Together, these coordinated modules preserve lysosomal integrity and prevent cathepsin release.
    Transport Escrt Adaptor Alix, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/pmc13038206-28-9-0
    Average 86 stars, based on 1 article reviews
    transport escrt adaptor alix - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    alix  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology alix
    ( A ) The <t>ESCRT</t> machinery, recruited by galectin-3 (Gal3) and <t>ALIX,</t> polymerizes at rupture sites to reseal small pores. ( B ) The PI4K2A/ORP/ATG9A lipid-transfer axis forms ER-lysosome bridges that restore membrane lipid composition and curvature. ATG2 cooperates with ATG9A vesicles to deliver lipids for bilayer expansion. ( C ) Neutral sphingomyelinase–dependent conversion of sphingomyelin to ceramide promotes local fusion and sealing. ( D ) Under severe stress, protein-RNA condensates assemble into transient stress granule patches that shield lesions until structural repair completes. Together, these coordinated modules preserve lysosomal integrity and prevent cathepsin release.
    Alix, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Alix/pmc12874330-260-38-42
    Average 96 stars, based on 1 article reviews
    alix - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins (ALIX, TSG101, and CD81) and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.

    Journal: Bioactive Materials

    Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells

    doi: 10.1016/j.bioactmat.2026.02.029

    Figure Lengend Snippet: Preparation and characterization of engineered ADGRG1-targeting and hypoxia-treated EVs. (A)Induced fit docking analysis of ADGRG1-binding peptide (A1TP, 7 peptides) and extracellular domain of ADGRG1 protein (PDB database: 7SF8). (B) Analysis of the binding of the A1TP to purified ADGRG1 proteins using a microscale thermophoresis (MST) binding assay. (C) Induced fit docking analysis of A1TP-PEG and extracellular domain of ADGRG1 protein. (D) The binding free energy between A1TP or A1TP-PEG and ADGRG1 were calculated using molecular dynamics simulations. Lower values indicate more stable interactions, with values less than or equal to −20 considered as stable binding modes. (E) Schematic illustration of the conjugating reaction between DSPE-PEG-Alkyne and A1TP. Schematic illustration of the fabrication of A1TP-HX-EVs through external modification by A1TP anchoring. Specific steps for the synthesis of DSPE-PEG-A1TP (DPA) are shown in . (F) FT-IR analysis showed the characteristic peaks of the DSPE-PEG-A1TP. The new triazole ring itself showed a characteristic C=N stretching vibration, a peak at 1538 cm −1 revealed the successful conjugation of A1TP. (G) H Nuclear magnetic resonance (NMR) spectra of DSPE-PEG-A1TP in D2O. The hydrogen signatures of the phenyl and phenol groups at 7.5-8.0 ppm confirmed the successful conjugation of DSPE to A1TP. (H) Western blot analysis verified the presence of three EV marker proteins (ALIX, TSG101, and CD81) and one EV negative marker (GM130) in EVs, HX-EVs, and A1TP-HX-EVs. (I) Transmission electron microscopy (TEM) images of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 200 nm. (J) Zeta potentials of EVs, HX-EVs and A1TP-HX-EVs, n = 3. Two-tailed unpaired Student's t-test was used for statistical analysis. ns, not significant. A two-tailed unpaired Student's t-test was used for statistical analysis. (K) Representative images of the spherical morphology and dispersion states of EVs, HX-EVs and A1TP-HX-EVs. Scale bar, 500 nm. (L) Size distributions of EVs, HX-EVs and A1TP-HX-EVs.

    Article Snippet: Finally, the presence of the characteristic EV markers Alix (92880, Cell Signaling Technology), CD81 (56039, Cell Signaling Technology) and TSG101 (sc-7964, Santa Cruz Biotechnology) was confirmed by Western blot analysis.

    Techniques: Binding Assay, Purification, Microscale Thermophoresis, Modification, Conjugation Assay, Nuclear Magnetic Resonance, Western Blot, Marker, Transmission Assay, Electron Microscopy, Two Tailed Test, Dispersion

    Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. ALIX, ALG‐2‐interacting protein X; ApoA1, apolipoprotein A1; CD63, cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.

    Journal: Alzheimer's & Dementia : Translational Research & Clinical Interventions

    Article Title: A potential multimodal biomarker – cognitive signature associated with the conversion from subjective cognitive decline to mild cognitive impairment

    doi: 10.1002/trc2.70240

    Figure Lengend Snippet: Characterization and imaging of serum‐derived cEVs. (A) cEVs images as acquired by transmission electron microscopy; bar represents 100 nm. (B) Concentration and size distribution of cEVs examined by NTA using Nanosight‐NS300. (C) ApoA1 concentration in serum and cEVs. (D) Immunoblot detection of EV protein markers and non‐associated proteins. The parametric Student's t ‐test was used for statistical analysis of ApoA1; *** p < 0.001. ALIX, ALG‐2‐interacting protein X; ApoA1, apolipoprotein A1; CD63, cluster of differentiation 63; cEVs, circulating extracellular vesicles; CTR, controls; nm, nanometer; NTA, nanoparticle tracking analysis; SCD, subjective cognitive decline; TSG101, tumor susceptibility gene 101.

    Article Snippet: Proteins were incubated for 30 min with primary antibodies against TSG101 (Novus Biologicals LLC, Catalog No.: NB200–112, 1:10), CD63 (R&D Systems; Catalog No.: MAB50482, 1:20), ALIX (R&D Systems; Catalog No.: MAB50482, 1:20), and calnexin (Novus Biologicals LLC; Catalog No.: NB100–1965, 1:25), followed by washing and incubation with species‐specific secondary antibodies.

    Techniques: Imaging, Derivative Assay, Transmission Assay, Electron Microscopy, Concentration Assay, Western Blot

    ( A ) The ESCRT machinery, recruited by galectin-3 (Gal3) and ALIX, polymerizes at rupture sites to reseal small pores. ( B ) The PI4K2A/ORP/ATG9A lipid-transfer axis forms ER-lysosome bridges that restore membrane lipid composition and curvature. ATG2 cooperates with ATG9A vesicles to deliver lipids for bilayer expansion. ( C ) Neutral sphingomyelinase–dependent conversion of sphingomyelin to ceramide promotes local fusion and sealing. ( D ) Under severe stress, protein-RNA condensates assemble into transient stress granule patches that shield lesions until structural repair completes. Together, these coordinated modules preserve lysosomal integrity and prevent cathepsin release.

    Journal: The Journal of Clinical Investigation

    Article Title: Lysosomal homeostasis at the crossroads of neurodegeneration

    doi: 10.1172/JCI199845

    Figure Lengend Snippet: ( A ) The ESCRT machinery, recruited by galectin-3 (Gal3) and ALIX, polymerizes at rupture sites to reseal small pores. ( B ) The PI4K2A/ORP/ATG9A lipid-transfer axis forms ER-lysosome bridges that restore membrane lipid composition and curvature. ATG2 cooperates with ATG9A vesicles to deliver lipids for bilayer expansion. ( C ) Neutral sphingomyelinase–dependent conversion of sphingomyelin to ceramide promotes local fusion and sealing. ( D ) Under severe stress, protein-RNA condensates assemble into transient stress granule patches that shield lesions until structural repair completes. Together, these coordinated modules preserve lysosomal integrity and prevent cathepsin release.

    Article Snippet: Galectin-3 interacts with the endosomal sorting complex required for transport (ESCRT) adaptor ALIX and TSG101 to nucleate ESCRT-III filaments that constrict and seal the membrane leak ( , ).

    Techniques: Membrane